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undergraduate thesis
Izolacija metalotioneina upotrebom gel filtracije
2019. urn:nbn:hr:182:996665
Lujanac, Josip
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Department of Analytical, Organic and Applied Chemistry

Cite this document

Lujanac, J. (2019). Izolacija metalotioneina upotrebom gel filtracije (Undergraduate thesis). Retrieved from https://urn.nsk.hr/urn:nbn:hr:182:996665

Lujanac, Josip. "Izolacija metalotioneina upotrebom gel filtracije." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:182:996665

Lujanac, Josip. "Izolacija metalotioneina upotrebom gel filtracije." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2019. https://urn.nsk.hr/urn:nbn:hr:182:996665

Lujanac, J. (2019). 'Izolacija metalotioneina upotrebom gel filtracije', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, accessed 15 March 2023, https://urn.nsk.hr/urn:nbn:hr:182:996665

Lujanac J. Izolacija metalotioneina upotrebom gel filtracije [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry; 2019 [cited 2023 March 15] Available at: https://urn.nsk.hr/urn:nbn:hr:182:996665

J. Lujanac, "Izolacija metalotioneina upotrebom gel filtracije", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:182:996665

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Metadata
TitleIzolacija metalotioneina upotrebom gel filtracije
Title (english)Isolation of metallothioneins using gel filtration
AuthorJosip Lujanac
MentorMartina Šrajer Gajdošik (mentor)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
(Department of Analytical, Organic and Applied Chemistry)
Osijek
Defense date and country2019-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
Metalotioneini su proteini male molekulske mase i specifične strukture bogate cisteinima. Prisutni su u mnogim organizmima te su važni za održavanje homeostaze esencijalnih metalnih iona te za detoksifikaciju teških metala. Njihova detekcija i analiza zahtjeva prethodnu izolaciju iz kompleksnih bioloških uzoraka. Gel filtracija je vrsta tekućinske kromatografija kojom se proteini razdvajaju na osnovi veličine te je pogodna za izdvajanje malih proteina, poput metalotioneina, iz kompleksnih bioloških uzoraka. U ovom su radu, metodom gel filtracije uz Sephadex G-50 i G-100, izdvojeni metalotioneini iz homogenata gujavica (Lumbricus terrestris) tretiranih cinkom.
Abstract (english)
Metallothioneins are low molecular weight proteins with specific structure rich in cysteines. They are present in many organisms and are important for maintaining homeostasis of essential metal ions and for detoxification of heavy metals. Their detection and analysis requires prior isolation from complex biological samples. Gel filtration is a type of liquid chromatography that separates proteins based on their size and is suitable for separating small proteins, such as metallothionein, from complex biological samples. In this work, gel filtration with Sephadex G-50 and G-100 was used for isolation of metallothioneins from zinc-treated earth worm (Lumbricus terrestris) homogenates.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:182:996665
Study programmeTitle: Chemistry
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2019-12-05 10:50:01