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master's thesis
Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu
2021. urn:nbn:hr:182:773221
Kassab, Joud
Josip Juraj Strossmayer University of Osijek
Department of Chemistry
Department of Analytical, Organic and Applied Chemistry

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Kassab, J. (2021). Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:182:773221

Kassab, Joud. "Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:182:773221

Kassab, Joud. "Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:182:773221

Kassab, J. (2021). 'Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, accessed 07 December 2024, https://urn.nsk.hr/urn:nbn:hr:182:773221

Kassab J. Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of Chemistry; 2021 [cited 2024 December 07] Available at: https://urn.nsk.hr/urn:nbn:hr:182:773221

J. Kassab, "Primjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:182:773221

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Metadata
TitlePrimjena plinske kromatografije (GC) za određivanje ostatnog otapala u melatoninu
Title (english)The application of gas chromatography (GC) for determination of residual solvent in melatonin
AuthorJoud Kassab
MentorAna Amić (mentor)
Committee memberOlivera Galović (predsjednik povjerenstva)
Committee memberAna Amić (član povjerenstva)
Committee memberMartina Šrajer Gajdošik (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Department of Chemistry
(Department of Analytical, Organic and Applied Chemistry)
Osijek
Defense date and country2021-07-23, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Analytic Chemistry
Abstract
U ovom radu provedeni su razvoj i validacija HSS-GC-FID metode za određivanje ostatnih otapala (metanola, 2-propanola, tert-butil-metil-etera i etil acetata) u sirovini melatonina. Melatonin je hormon epifize s brojnim biološkim učincima na organizam pa se sintetski melatonin često koristi kao aktivna tvar u pripremi farmaceutskih preparata. Ključni parametri validacije bili su specifičnost, linearnost, raspon, preciznost, točnost, stabilnost te limit (granica) detekcije i kvantifikacije. Metoda je validairana prema ICH smjernicama i određene su kvantifikacijske i detekcijske granice za svako otapalo. Rezultati su pokazali da je metoda visoko specifična (selektivna), linearna, točna i precizna unutar ispitivanog područja. Otopine standarda i uzorka stabilne su 2 dana na sobnoj temperaturi i u hladnjaku. S obzirom da su zadovoljeni kriteriji prihvatljivosti svih validacijskih parametara, može se zaključiti da je razvijena HSS-GC-FID metoda prikladna za određivanje ostatnih organskih otapala u sirovini melatonina.
Abstract (english)
In this thesis HSS-GC-FID method for determination of residual solvents (methanol, 2-propanol, tert-butyl methyl ether and ethyl acetate) in melatonin was developed and validated. Melatonin is a hormone secreted by the human pineal gland and has many various effects on the body, hence synthetic melatonin is used as an active component in the preparation of pharmaceutical products. Key validation parameters were selectivity, linearity, range, precision, accuracy, stability, and limits of detection and quantification. Method has been validated in accordance with ICH guidelines and quantification and detection limit for all studied solvents was determined. Results of validation experiments confirmed that this method is highly selective, linear in working range, accurate and precise. Standard and sample solutions were stable for two days at room temperature and in the fridge. Considering all conditions for validation were met, it can be concluded that developed HSS-GC-FID method for the determination of residual organic solvents in melatonin raw material is suitable for its intended purpose.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:182:773221
Study programmeTitle: Graduate University Study of Chemistry; research program; specializations in: Research program
Course: Research program
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-08-08 07:23:25