The aim of this graduate thesis is to state and explain the forensic methods for the analysis of the colors in the art works by describing and commenting on some examples. In painting, color is the most important element of the image. It is produces by physical mixing of binders and pigments with various additives that have the coloring properties of the surface to which they are applied. By means of forensic methods and their combination, it is possible to solve the question of authenticity of artwork, to determine the age and origin of images, to mitigate the consequences of decay of works of art, to determine the state of image, to analyze the surface of images, to reveal substructures, other layers of colors, to reveal false art etc. Methods that address these problems include: X – ray fluorescence spectroscopy (XRF), optical microscopy, scanning electron microscopy with energy dispersive X- ray spectroscopy (SEM-EDS), micro – chemical analysis, thin layer chromatography, infrared spectroscopy, infrared spectroscopy with Fourier transformation (FTIR spectroscopy), infrared reflection, Raman spectroscopy, optical fiber reflection spectroscopy (FORS), ultraviolet fluorescence (UVF), ultraviolet reflection and others. In the methodological part of the paper, the teaching unit ''Some methods of separating the mixture – CHROMATOGRAPHY'' was elaborated in the form of a block hour with the aim of introducing a method for separating clean substances from homogeneuos liquid or gaseous mixtures, i.e. chromatography.