Early discoveries showed that rapid consumption of glucose and secretion of lactate are associated with many types of cancer. Cancer is characterized by constant growth and expansion to other organs which it destroys (as well as it destroys its originating organ). Furthermore, many factors affect the localization of malignant cells; nicotine, various insecticides, microorganisms, also erroneous and over-nutrition are only some of them. Different experimental models showed that apoptosis is a fundamental process in maintaining the mass and functional abilities of certain organs. We can distinguish the outer pathway of apoptosis that involves death receptors and the inner pathway that is also called mitochondrial. Observation of O.Warburg is based on the cancer cells that have increased glycolytic activity and elevated levels of lactic acid even under aerobic conditions of the cell. Significantly two glycolytic enzymes, hexokinase (HK) and Glyceraldehyde 3-phosphate dehydrogenase (GADPH) act as crucial players in modulating the balance between cell survival and cell death, while M2 isoform of pyruvate kinase (PKM-2) supports anabolic metabolism and is expressed both in malignant cells and healthy tissue. Ongoing clinical trials will help assess the full potential of the inhibition of other glycolitic enzimes, like phosphofructokinase (PFK) and pyruvate kinase (PK), for the development of novel anticancer therapies.