Vitamin E is a fat-soluble vitamin and is an excellent biological antioxidant. It is extremely important for human health and is a key additive to animal feed due to the strengthening the immune status, improving food quality and thus increasing the intake of vitamin E in the human body. The aim of this study was to determine the content of vitamin E in eggs obtained from laying hens fed with feed mixtures enriched with functional ingredients using high-resolution liquid chromatography. The concentration of vitamin E was determined in eggs obtained from laying hens fed with a standard feed mixture, in eggs obtained from laying hens fed with feed mixture enriched with vitamin E, commercially available eggs and eggs obtained from laying hens from family farms. The prepared yolk samples were analyzed and the values of vitamin E concentrations were measured. The highest concentration of vitamin E, compared to the literature data, was measured in the group of laying eggs whose diet was enriched with vitamin E. Slightly lower concentration was measured in eggs obtained from family farms where hens are fed with variety of foods. The average value of vitamin E in laying hens fed with standard feed mixture was 34% lower compared to literature data. The lowest concentration was measured in commercially available eggs. The average value was significantly lower in relation to the data found in literature and to the data of measured values of other groups. A comparison of the obtained data showed that the addition of vitamin E to the feed of laying hens will increase its content in eggs. It is also evident that the content of vitamin E in eggs depends on the food on which the laying hens are fed.