Capillary electrophoresis (CE) is a highly efficient separation technique for quantitation of different charged compounds. Electrophoresis on microchip (MCE) is a miniaturized version of the CE.Alkali and earthalkali metals were routinely monitored in a variety of aqueous samples important to several industries, such as drinking and waste water industries.In this investigations a MCE device with capacitively coupled contactless conductivity detection (engl. Capacitively-Coupled Contactless Conductivity Detection, CCCCD, C4D) has been successfully applied for analysis of several cations, individually and in mixture. The separation was carried out on a borosilicate microchip with the separation channel length of 4 mm. Acetic acid solution of the concentration 0.5 M has been used as a background electrolyte (pH = 2.5). The applied injection voltage was +0.7 kV and the separation voltage was +1.4 kV. The influence of the following parameters on the separation ability was investigated: injection time, injection voltage, separation time and separation voltage. The linear response region for each particular cation investigated, both single and in a mixture, has been determined using linear regression. The method was applied to determine cations in tap water, mineral water and rain water.In the teaching part of the thesis it is proposed a preparation lecture called The basic properties of liquids. Lesson is designed as a combination of frontal lectures and students group work with experiments. Aim of the group work is to achieve the independence of students, and experiments are helpfull for thinking about teaching materials and to make conclusions.