Fats are organic compounds which belong to a broader group of compounds called lipids. They belong to a group of compounds that are soluble in organic solvents. Fats are, according to the chemical composition, glycerol and higher fatty acids esters and are, therefore, called triglycerides. Fats have a significant effect on the proportion of cholesterol in the blood. Saturated and trans fatty acids, although belonging to nonsaturated fatty acids, increase the share of „bad“ cholesterol, LDL (Low Density Lipoprotein) cholesterol, and at the same time decrease the share of „good“ cholesterol, HDL (High Density Lipoprotein) cholesterol. Mono and polyunsaturated fatty acids increase the share of „good“ cholesterol and decrease the share of „bad“ cholesterol. Soxhlet method is common method for determining free fats in samples. Using this method, fat was extracted in the samples of hens feed and the present amount of fat was determined. The extraction was carried out with a suitable solvent: petroleum ether. Fats are a good source of energy in the body and have good energy contribution, fats are essential part of the diet because they provide to the body essential fatty acids. It is important, in nutrition of poultry, to provide food rich in polyunsaturated fatty acids due to their useful effect on the organism. The aim of this study is, to determine the fat content in feed for laying hens. using Soxhlet extraction method.